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Friday, April 24, 2020 | History

2 edition of DNA topoisomerase II[alpha] found in the catalog.

DNA topoisomerase II[alpha]

John Peter McPherson

DNA topoisomerase II[alpha]

role in resistance to antineoplastic agents and induction of apoptosis.

by John Peter McPherson

  • 86 Want to read
  • 18 Currently reading

Published .
Written in English


The Physical Object
Pagination177 leaves.
Number of Pages177
ID Numbers
Open LibraryOL19149813M
ISBN 10061235248X

B Anti-TOP2A Antibody detects endogenous levels of total TOP2A protein. Validated for WB, IHC, and ELISA. Reactive for Human. AssayBiotechnology an original antibody g: book.


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DNA topoisomerase II[alpha] by John Peter McPherson Download PDF EPUB FB2

DNA topoisomerases represent an essential family of DNA processing enzymes and a large number of topoisomerase inhibitors are used clinically for the treatment of various human cancers.

Novels drugs are in clinical development both against type I and type II topoisomerases. The book will include. DNA Topoisomerase Protocols, II: Enzymology and Drugs brings together an unprecedented collection of cutting-edge experimental protocols for investigating the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial drugs.

The IUPHAR/BPS Guide to Pharmacology. DNA topoisomerase II DNA topoisomerase II[alpha] book - DNA topoisomerases. Detailed annotation on the structure, function, physiology, pharmacology and clinical relevance of drug targets. Type II topoisomerases are required for the management of DNA superhelicity and chromosome segregation, and serve as frontline targets for a variety of small-molecule therapeutics.

To better understand how these enzymes act in both contexts, we determined the Å-resolution structure of the DNA cleavage core of human topoisomerase IIα Cited by: A Anti-TOP2A (Phospho-Ser) polyclonal Antibody detects TOP2A only when phosphorylated at Ser Validated for WB, IHC and ELISA.

Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody g: book. Introduction. Topoisomerases are essential enzymes that manage the higher-order structural state of DNA. By selectively cleaving, rearranging, and religating DNA strands, topoisomerases can both alter the superhelical state of DNA and help disentangle interlinked chromosomes.

1 Topoisomerases fall into two general categories, termed type I or type II, depending on whether one or both DNA Cited by: Ensembl ENSG ENSMUSG UniProt Q Q RefSeq (mRNA) NM_ NM_ NM_ RefSeq (protein) NP_ NP_ NP_ Location (UCSC) Chr 3: – Mb Chr – Mb PubMed search Wikidata View/Edit Human View/Edit Mouse DNA topoisomerase 2-beta is an enzyme that in Aliases: DNA topoisomerase II[alpha] book, TOPIIB, top2beta.

Coutts J, Plumb JA, Brown R, Keith WN. Expression of Topoisomerase-Ii-Alpha and Topoisomerase-Ii-Beta in an Adenocarcinoma Cell-Line Carrying Amplified Topoisomerase-Ii-Alpha and Retinoic Acid Receptor-Alpha Genes.

British Journal of Cancer. ; – [PMC free article] [Google Scholar]Cited by: In, Topoisomerase II – DNA’s Natural Detangler, you will learn how a little enzyme, known as Topoisomerase II, keeps our that from happening. $ Availability. Topoisomerase II isoforms.

Whereas lower eukaryotes such as yeast and Drosophila encode only a single type II topoisomerase [23,24], vertebrate species express two discrete forms of the enzyme, topoisomerase IIα and IIβ [14,19,25,26].These enzymes display a high degree of amino acid sequence identity (~70%), however, they differ in their protomer Cited by: Serine is a major site of phosphorylation on human topoisomerase II alpha protein in vivo and is a substrate for casein kinase II in vitro.

J Biol Chem ; PubMed; Li H, Wang Y, Liu X. Plk1-dependent phosphorylation regulates functions of DNA topoisomerase II alpha in cell cycle progression. J Biol Chem ; Missing: book. Human topoisomerase II beta is prepared by overexpressing in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods developed enzyme is supplied at a concentration of U/μl in Dilution g: book.

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication.

Eubacterial DNA topoisomerase IV (E. DNA topoisomerase II[alpha] book IIA C2E2 hetero- ParC, tetramer ParE, Yeast DNA topoisomerase II (S.

cerevisiae) IIA Homodimer Mammalian DNA topoisomerase IIfi(human) IIA Homodimer Mammalian DNA topoisomerase IIfl(human) IIA Homodimer Archaeal DNA topoisomerase VI (Sulfolobus IIB A2B2 hetero- A, File Size: 1MB. Human Topoisomerase II Assay Kit Product Description.

This kit is designed to allow quick and specific assays for eukaryotic type II DNA topoisomerases. This kit facilitates the purification and characterization of type II enzymes and contains all reagents necessary for routine assays of type II g: book.

A companion volume, DNA Topoisomerase Protocols, v. 2: Enzymology and Drugs, provides detailed protocols to study the catalytic activities of DNA topoisomerases, as well as their specific interactions with topoisomerase-targeted antitumor and antibacterial : Hardcover.

DNA Topoisomerase Protocols: Volume II: Enzymology and Drugs (Methods in Molecular Biology) (v. 2): Medicine & Health Science Books @ Discovery. In the s, James C. Wang was the first to discover a topoisomerase when he identified E. coli topoisomerase I. Topo EC-codes are as follows: type I, EC ; type II: EC Function.

The overall function of DNA topoisomerase is to manage the topological state of the DNA in the cell. There are two types or families of this enzyme; type I family and type II Missing: book. Fission Yeast DNA topoisomerase II ( kD) consists of an enzymatically active kD core, approximately kD NH2-terminal and kD COOH-terminal domains.

The question addressed in the present study is what is the role of the topo II by: Higher Expression of Topoisomerase II Alpha Is an Independent Marker of Increased Risk of Cancer-specific Death in Patients with Clear Cell Renal Cell Carcinoma Alexander S. Parker, a, * Jeanette E.

Eckel-Passow, b Daniel Serie, a Tracy Hilton, a Mansi Parasramka, c Richard Joseph, d Kevin Wu, e John C. Cheville, f and Bradley C Cited by: DNA topoisomerases are a group of enzymes involved in the topology of DNA during critical cellular processes including replication, recombination, transcription, and repair systems by transiently breaking one or two strands of DNA (Bodley and Liu, Cited by:   DNA topoisomerase II alpha is the major chromosome protein recognized by the mitotic phosphoprotein antibody MPM Proc Natl Acad Sci U S A.

Sep 15; 90 (18)– [PMC free article] Takano H, Kohno K, Ono M, Uchida Y, Kuwano M. Increased phosphorylation of DNA topoisomerase II in etoposide-resistant mutants of human cancer KB Cited by: Type II topoisomerases (TOP2s) resolve the topological problems of DNA by transiently cleaving both strands of a DNA duplex to form a cleavage complex through which another DNA segment can be transported.

Several widely prescribed anticancer drugs in Cited by:   Topoisomerases are enzymes that wind and unwind DNA to facilitate protein synthesis and DNA replication (Wikipedia: Topoisomerase [Accessed 10 August ]) Topoisomerase IIA cuts both strands of the DNA helix to unwind them (Wikipedia: Topoisomerase II [Accessed 10 August ]) Expression is predictive of prognosis in.

J Deffie AM, et al., Evidence for a mutant allele of the gene for DNA topoisomerase II in adriamycin-resistant P murine leukemia cells [published erratum appears in Cancer Res Jan 15;50(2)]. Cancer Res. Dec 15;49(24 Pt 1)   The type II topoisomerases are important enzymes that are involved in maintaining the structure of DNA and chromosome segregation during both replication and transcription of DNA.

One of these enzymes, topoisomerase II alpha, is involved in the replication of DNA and cell proliferation, and is highly expressed in rapidly dividing cancer cells.

UNIVERSITY OF CALGARY Characterization of Salicylates as Novel Catalytic Inhibitors of Human DNA Topoisomerase II Alpha by Jason Theodore Bau A THESIS SUBMITTED TO THE FACULTY OF GRADUATE STUDIES IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MEDICAL.

The Ecoli topoisomerase I is a holoenzyme with three Zn (II) atoms in the tetracysteine motifs near its carboxy terminus. It is 97 kDa in weight. Topoisomerase I has several unusual features.

It does not need ATP hydrolyzing to catalyze the topological rearrangement of DNA. The exiting feature of the topoisomerase I is, it is a fully functional Missing: book. - type II topoisomerase in E. Coli-can introduce negative supercoils into the DNA ahead of the replication fork.

- in the absence of DNA gyrase, strand separation would cause overwinding of the DNA ahead of the replication fork, a generating positive supercoils that would hinder DNA. Mammals have two isoenzymes topII-alpha and beta. In the presence of magnesium ions, the tyrosine from each top II monomer attacks the phosphodiester bond four bases apart on opposite strands of the G duplex and becomes covalently linked to the 5' end of the broken DNA.

Topoisomerase II alpha (TOP2A) is encoded by the TOP2A gene located on chromosome 17qq It is a key nuclear enzyme for controlling of topological states of DNA by generating transient breakage in double-stranded DNA; therefore it is involved in processes such as DNA replication and transcription, and chromosome formation, enrichment, and Cited by: 7.

Watt and Hickson () reviewed in extenso the structure and function of type II DNA topoisomerases. Mondal and Parvin () demonstrated that DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation.

Specific inhibitors of topoisomerase II blocked transcription on chromatin. DNA Topoisomerase (ATP Hydrolysing) Topoisomerase II is an essential enzyme that acts as a dimer to relieve superhelical stress, and the topoisomerase II poisons, which are used to treat nearly one-half of all cancers, lock the complex in the open-gate conformation, creating a persistent protein-associated DSB [].

Ubiquitin-specific protease 15 (USP15) is a widely expressed deubiquitylase that has been implicated in diverse cellular processes in cancer. Here we identify topoisomerase II (TOP2A) as a novel Cited by: 8. The camptothecins are topoisomerase I inhibitors, whereas the podophyllotoxins are topoisomerase II omerase is an enzyme that cuts and reseals DNA strands, a process that is essential for DNA synthesis.

During the process of DNA replication or transcription, a significant amount of torsional strain is placed on the DNA helix. DNA. Teniposide, another topoisomerase II inhibitor, is more active against the alpha form. Etoposide is times less active against the beta form than the alpha. E) Etoposide stabilizes the cleavable complex of DNA and topoisomerase.

There is an accumulation of cells in S and G 2 phases. Single and double strand breaks appear in DNA Missing: book.

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA.

The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA.

Book Review () more Subjects. Subjects. Sort by Item Count. Add All Remove All dna topoisomerases, type ii - metabolism () Filter by: Remove filter: dna repair () Topoisomerase (topo) II alpha and II beta maintain genome stability and are targets for anti-tumor drugs. In this study, we demonstrate that the.

DNA gyrase is a bacterial type II DNA topoisomerase with a tetrameric structure composed of two A subunits, the kDa proteins encoded by the gyrA (formerly nalA) gene, and two B subunits, the k Da proteins encoded by the gyrB (formerly cou) gene (reviewed by Cozzarelli, ; Gellert, ; Sutcliffe et al., ; Wang, ).Mechanistic studies and electron.

In molecular biology Type I topoisomerases are enzymes that cut one of the two strands of double-stranded DNA, relax the strand, and reanneal the strand. They are further subdivided into two structurally and mechanistically distinct topoisomerases: type IA and type IB. Type IA topoisomerases change the linking number of a circular DNA strand by units of strictly ro: IPR.

DNA topoisomerase or simply Topoisomerase (ECtype I DNA topoisomerase, untwisting enzyme, relaxing enzyme, nicking-closing enzyme, swivelase, omega-protein, deoxyribonucleate topoisomerase, DNA gyrase) is an enzyme with systematic name DNA topoisomerase.

This enzyme catalyzes the following chemical reactionBRENDA: BRENDA entry.Immunohistochemical study of DNA topoisomerase I, DNA topoisomerase II alpha, p53, and Ki in oral preneoplastic lesions and oral squamous cell carcinomas.DNA topoisomerase 2 alpha (TOP2A) is an essential nuclear enzyme, required for resolution of topological stress associated with DNA replication.

TOP2A introduces transient double strand breaks (DSB) on DNA in an ATP-dependent fashion to allow changes in DNA topology and eliminate over-winding [ 1, 2 ].Cited by: 7.